Effect of Enzymes on Starch

Trials recorded before and after of agars (starch) in different concentration of amylase
Diameter of iodine circles (mm) in different concentrations
0 M 0.1 M 1 M 10 M
Trials Before (after 24h)
1 15 35 40 45
2 15 40 43 47
3 15 41 44 48
4 15 39 45 47
5 15 42 43 50
Average (mm) 15 39.4 43 51.4
Standard Deviation 0 ± 2.7 ± 1.87 ± 1.82
After (after 96h)
1 15 40 42 47
2 15 34 40 50
3 15 40 41 45
4 15 38 37 53
5 15 42 40 55
Average (mm) 15 38.8 40 50
Standard Deviation
0 ± 3.03 ± 1.87 ± 4.12

Qualitative data
Amylase is milky white in color
Petri Dishes contains condensed vapor on the lid
Lots more condensed vapor after taking out of incubator
Majority of the area is dark purple in color after pouring iodine on the agar

Sample Calculations

Average diameter
The aim of this lab is to compare the results of different concentrations of amylase to starch. In order to do so, we can examine the size of circles of each concentration section to determine the how effective each concentration is. To simplify the procedure of data processing, I decided to calculate the average diameter of iodine circles of each concentration section with the simple average calculation method of adding up the total diameters of 5 trials of concentration sections, then divide the total by 5.

Equation: total amount of data / number of sets of data

As such, to find the average diameter of trial 1:

((15+15+15+15+15)/5) mm = 15 mm

Standard deviation
By calculating the standard deviation of the sets of data, we can see how spread out the data is. The larger the standard deviation number, the more spread out the result, the more deviation from the mean. To calculate the standard deviation, we take the mean of the set of values of a trial and subtract it from each individual sets of petri dish trials. After doing so, square each of the results and add the values up and finally square root the answer.


A sample of trial 2 (96H) shows how I worked out...