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26.........Practical work

11. Determination of water potential of plant tissues

The water potential of a plant tissue can be determined by the following principle. If the tissue shows no net gain or loss of water when immersed in a solution of known molarity, its water potential is equal to that of the external solution. Samples of the tissue are allowed to come to equilibrium in a range of solutions of different concentrations. When the tissue shows neither an increase nor a decrease in mass or length, the water potential of the potato tissue is the same as that of the external solution. A. By length

Procedure 1. Use 1M sucrose solution and distilled water to prepare a series of 10 cm3 sucrose solutions in boiling tubes of different concentrations: 1M, 0.8M, 0.6M, 0.4M 0.2M and 0.0M. Label the boiling tubes. 2. Use a cork borer to obtain cylinders of potato tissue with the same diameter. Cut them all to the same length of 5 cm. It is important to work quickly to avoid loss of water through evaporation as this would lower the water potential of the tissue. 3. 4. Immerse two cylinders of potato tissue in each tube and cover the tubes with sealing film. Leave the set up for one hour.

Practical work.........27

5.

Remove the cylinders from each tube. Measure the length of the cylinders, and calculate the percentage change in length using the formula below: % change in length = final length – initial length initial length x 100%

6. 7.

Find the mean percentage change in length of the cylinders at each concentration. Plot a graph of the mean percentage change in length (vertical axis) against the concentration of sucrose solution (horizontal axis).

8.

From the graph, determine the concentration of sucrose solution which causes no change in length of the tissue.

9.

The water potential of the potato tissue can be expressed in terms of the molarity of sucrose solution that causes no change in length of the tissue.

B.

By weight...

11. Determination of water potential of plant tissues

The water potential of a plant tissue can be determined by the following principle. If the tissue shows no net gain or loss of water when immersed in a solution of known molarity, its water potential is equal to that of the external solution. Samples of the tissue are allowed to come to equilibrium in a range of solutions of different concentrations. When the tissue shows neither an increase nor a decrease in mass or length, the water potential of the potato tissue is the same as that of the external solution. A. By length

Procedure 1. Use 1M sucrose solution and distilled water to prepare a series of 10 cm3 sucrose solutions in boiling tubes of different concentrations: 1M, 0.8M, 0.6M, 0.4M 0.2M and 0.0M. Label the boiling tubes. 2. Use a cork borer to obtain cylinders of potato tissue with the same diameter. Cut them all to the same length of 5 cm. It is important to work quickly to avoid loss of water through evaporation as this would lower the water potential of the tissue. 3. 4. Immerse two cylinders of potato tissue in each tube and cover the tubes with sealing film. Leave the set up for one hour.

Practical work.........27

5.

Remove the cylinders from each tube. Measure the length of the cylinders, and calculate the percentage change in length using the formula below: % change in length = final length – initial length initial length x 100%

6. 7.

Find the mean percentage change in length of the cylinders at each concentration. Plot a graph of the mean percentage change in length (vertical axis) against the concentration of sucrose solution (horizontal axis).

8.

From the graph, determine the concentration of sucrose solution which causes no change in length of the tissue.

9.

The water potential of the potato tissue can be expressed in terms of the molarity of sucrose solution that causes no change in length of the tissue.

B.

By weight...

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