Biolegy Enzymes

1. What effect does the following have on enzymes.

Temperature – An increase in temperature would mean there is more kinetic energy transferred to the enzyme and the substrate. More kinetic energy results in more collisions between the enzyme and the substrate. Which would increase the number of successful collisions. Therefore an increase in temperature increases the rate at which enzyme substrate complexes form. However when a enzyme and substrate reach the peek of there temperature it will become de natured and not work.

pH – Changing the pH compound that contains an enzyme will decrease the efficiency of the enzyme   because all enzymes have a specific optimum pH when it works and any different it has a chance to denature the enzyme.

Substrate concentration -   The higher the Substrate concentration the faster the rate of reaction will be because there are more substrates which means there is a higher chance to collide with a enzyme until enzyme saturation is reached. The lower the Substrate concentration means the rate of reaction will decrease because there are less substrates so the possible collisions decrease.

Enzyme concentration – the higher the Enzyme concentration the faster the rate of reaction because there are more enzymes in the solution so there is a greater chance of colliding into a substrate but if there are too high of enzymes to substrate ratio then when all the substrates are tacken then there will be unoccupied enzymes. If the concentration is decreased then there will be less chance of possible collisions.

Inhibitors – (I do not understand it)

1st method
Measure the time taken for amylase to completely break down starch, by withdrawing samples at 10 second intervals and noting the time at which the solution no longer gives a blue-black colour with iodine solution (but the iodine solution remains orange). Use buffers to provide solutions at different pHs. Calculate the rate of this enzyme controlled reaction by...