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Centrifuge and Spectrophotometer

  • Date Submitted: 04/19/2015 10:33 AM
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      1.1 Centrifugation Technique in Biochemistry
In this experiment, we have to know and understand the principle of centrifugation. We were also exposed to the functions of centrifuges and all of the group members were supposed to understand the Relative Centrifugal Force (RCF) and apply it in doing experiment or solving problem.
Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. The theoretical basis of this technique is the effect of gravity on particles (including macromolecules) in suspension. Two particles of different masses will settle in a tube at different rates in response to gravity. Centrifugal force, is used to increase this settling rate in an instrument called a centrifuge.
Centrifuge is a piece of equipment that puts an object in rotation around a fixed axis, applying a potentially strong force perpendicular to the axis of spin (outward). The centrifuge works using the sedimentation principle, where the centripetal acceleration causes denser substances and particles to move outward in the radial direction. At the same time, objects that are less dense are displaced and move to the centre.
Figure below shows one of the centrifuge commonly used in laboratory.
Figure 1.1

1.2   Colorimetry and Spectrophotometry Method
In this experiment, we need to understand the principal of colorimetry and spectrophotometry. We were also taught how to operate the spectrophotometry and reading the sample’s absorbance correctly. All of the group members were also needed to understand the Beer-Lambert’s law.
Colorimeter is a device used in colorimetry. In scientific fields the word generally refers to the device that measures the absorbance of particular wavelengths of light by a specific solution. This device is most commonly used to determine the concentration of a known solute in a given solution by the application of the Beer-Lambert law. A colorimeter is a device used to test the...


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