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Protein Electrophoresis Market - Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2015 – 2023

  • Date Submitted: 09/21/2015 03:16 AM
  • Flesch-Kincaid Score: 30 
  • Words: 745
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Proteins are biological macromolecules considered as the basic component of a majority of enzymes and other complex bimolecular compounds, and are also required in the synthesis of such compounds. Proteins differ from one another based on the number of peptide structures present within them and they way in which they are folded ore arranged. These peptides are further composed of amino acid molecules that together form a chain to give rise to the peptide’s respective structure. Protein electrophoresis has been a fundamental research process to evaluate the contents of a protein based on their molecular weight and their charge. Protein electrophoresis can either be done by either denaturing or non denaturing methods. Denaturing process allows protein unfolding and study of various polypeptides by their molecular weight and charge (charge to mass ratio), whereas non denaturing methods (also known as native gel methods) take protein size into consideration as a whole without unfolding the main structure thereby studying its shape and size in the process. Polyacrylamide gel is a commonly used porous gelling agent, for both methods, that allows for movement of proteins within the buffer medium. Denaturing methods employ sodium dodecyl suphate (SDS), a commonly used detergent agent that allows for unfolding of proteins into their polypeptides and in the process assists free movement of ionized protein molecules in the water medium. Together SDS polyacryilamide gel electrophoresis (SDS-PAGE) is the most common denaturing gel method for protein electrophoresis. Among native PAGE methods blue native and clear native PAGE methods are most common and inexpensive procedures, they are followed by the highly accurate quantitative preparative native continuous PAGE (QPNC-PAGE) that employs Nuclear Magnetic Resonance spectrometer to study molecules of protein polypeptide. Blue native (BN-PAGE) process requires the assistance of a Coomassie brilliant blue dye that provides charges...

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