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Bio 362 Exam Iii Solutions

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BIO 362 Exam III Solutions
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BIO 362 Exam III Solutions
 
 
Question 1.
Given the following DNA template strand of reporter gene (a gene used to report on mRNA and
protein expression):
 
5-CTATACTCCTCCATGTCTTACGTGGTAGTGGTGGTAGTGTACATCAAAAAAAAA-3
 
 
a) Write the likely mRNA sequence of this gene when it is expressed in bacteria.
 
 
b) Write the anticodon sequence of all tRNAs that could decode this mRNA (without Inosine
modifications in the tRNAs). Please make sure to indicate the correct directionality of
the codons and anticodons.
 
 
c) What is the minimum number of tRNAs required to decode this mRNA? Explain your
answer.
 
 
1
Bio362
Exam III
Name
 
d) Write the likely amino acids sequence of the protein produced from this gene.
 
 
e) Which approach/technique will you use to detect this mRNA? Briefly explain how this
approach works and what material/reagents you will need.
 
 
f) Which approach/technique will you use to detect this protein? Briefly explain how this
approach works and what material/reagents you will need.
 
 
2
 
 
g) You have discovered a new viral protein called Snoop that is a sequence specific mRNA
modification enzyme. When you express Snoop in bacteria or eukaryotes, along with your
reporter gene (shown above), you notice that you are unable to detect the reporter mRNA or
its protein product.
 
i.
Explain why there is no detectable reporter mRNA or protein when Snoop is
expressed in bacterial.
 
 
ii.
Explain which genes you will delete to stabilize the reporter mRNA and protein to
the effects of Snoop expression in bacteria?
 
 
iii.
Explain why there is no detectable reporter mRNA or protein when Snoop is
expressed in eukaryotes.
 
 
Question 2. Please provide brief definitions for the following terms (no more than one or two
sentences:
a. Attenuation
b. RRF
c. tmRNA
d. SspB
e. Molecular mimicry (Name one...

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