Ap Biology Enzyme Catalyse Lab

Introduction
The purpose of this lab is to use a chemical titration to measure and then calculate the rate of conversion of hydrogen peroxide (H2O2) to water and oxygen gas using the enzyme Catalase. I used forceps and scissors, then cut a small piece of liver and then place it in the test tube. Throughout the investigation, we will observe and estimate the reaction rate. I will then determine if it is endothermic or exothermic. I believe that the reactions rate will be that highest during the first interval (0-10 seconds) because there are larger amounts of substrate molecules than enzyme molecules, but as times increase the lower the reaction rate because there will be more product molecules as result of the chemical reactions between the enzyme and the substrate.
Materials
  * 6 Test tubes and test tube holders
  * 10-mL Graduated Cylinder
  * 40-mL of 3% Hydrogen peroxide solution
  * Straight-edged razor blade
  * Scissors and Tweezers
  * Thermometer
  * Stirring Rod
  * pH paper
  * Fresh Liver, Chicken Meat, Apple, and Potato

Methods

Part A
  1. Place 2 mL of the 3% Hydrogen Peroxide solution into a clean test tube.
  2. Using tweezers and scissors, cut a small piece of liver and add it to the test tube.
  3. Push the hydrogen peroxide with a stirring rod.
  4. Observe the bubbles.

Part B
  1. Place 2 mL of Hydrogen Peroxide in each of three clean test tubes.
  2. Then add each of the three test substances to the test tubes.
  3. Add a small piece of potato to the first tube.
  4. Add a small piece of chicken to the second tube.
  5. Add a small piece of apple to the last tube.
  6. Record the reaction rate of each substance for each test tube on a scale of 1-5, 5 being the fastest.
Part C
  1. Put a piece of liver into the bottom of a clean test tube.
  2. Cover the liver with a small amount of distilled water.
  3. Place the test tube into a boiling water bath for 5 minutes.
  4. Remove the test...